Sperm chromatin analysis by flow cytometry

Our group has experience using different techniques for assessing sperm chromatin status in different species (fish, amphibians, mammals). We have used the Comet assay, SCD (Halomax, Halosperm, etc.), SCSA and TUNEL. Currently, we are routinely carrying out SCSA analyses in spermatozoa from several species, including human.

We are open to collaborations with other groups that would be interested in analysing the sperm chromatin but currently do not have the time or resources to carry it out. We are collaborating with several groups that simply store their samples frozen and ship them to our lab from time to time, in order to perform the analyses.

Contact Dr. F. Martínez-Pastor for more information.