Full papers

This is a list of our recent publications as listed in a PubMed search.

For an alternative list, http://www.researcherid.com/rid/B-4277-2008.

  1. Gardela, J, Ruiz-Conca, M, Palomares, A, Olvera-Maneu, S, García-Calvo, L, López-Béjar, M et al.. Effect of Honey, Coenzyme Q10, and β-Carotene/α-Tocopherol as Novel Additives in Rabbit-Sperm Cryopreservation Extender. Animals (Basel). 2023;13 (14):. doi: 10.3390/ani13142392. PubMed PMID:37508170 PubMed Central PMC10376550.
  2. Blanco-Prieto, O, Mislei, B, Martínez-Pastor, F, Spinaci, M, Mari, G, Bucci, D et al.. Study of mitochondrial function in thawed bull spermatozoa using selective electron transfer chain inhibitors. Theriogenology. 2023;208 :8-14. doi: 10.1016/j.theriogenology.2023.05.021. PubMed PMID:37290146 .
  3. Caamaño, JN, Santiago-Moreno, J, Martínez-Pastor, F, Tamargo, C, Salman, A, Fernández, Á et al.. Use of the flavonoid taxifolin for sperm cryopreservation from the threatened Bermeya goat breed. Theriogenology. 2023;206 :18-27. doi: 10.1016/j.theriogenology.2023.05.004. PubMed PMID:37172535 .
  4. Tamargo, C, Salman, A, Caamaño, JN, Martínez-Pastor, F, Fernández, Á, Muiño, R et al.. Characterization of the Germplasm Bank for the Spanish Autochthonous Bull Breed "Asturiana de la Montaña". Animals (Basel). 2023;13 (8):. doi: 10.3390/ani13081402. PubMed PMID:37106966 PubMed Central PMC10135365.
  5. Lacalle, E, Martínez-Martínez, S, Fernández-Alegre, E, Soriano-Úbeda, C, Morrell, JM, Martínez-Pastor, F et al.. Low-density colloid centrifugation removes bacteria from boar semen doses after spiking with selected species. Res Vet Sci. 2023;158 :215-225. doi: 10.1016/j.rvsc.2023.03.024. PubMed PMID:37031470 .
  6. Salman, A, Fernández-Alegre, E, Francisco-Vázquez, R, Gómez-Martín, R, Fernández-Fernández, A, Areán-Dablanca, H et al.. Extension of the equilibration period up to 24 h maintains the post-thawing quality of Holstein bull semen frozen with OPTIXcell®. Anim Reprod Sci. 2023;250 :107209. doi: 10.1016/j.anireprosci.2023.107209. PubMed PMID:36889162 .
  7. Lacalle, E, Fernández-Alegre, E, Soriano-Úbeda, C, Martínez-Martínez, S, Domínguez, JC, González-Montaña, JR et al.. Single layer centrifugation (SLC) for bacterial removal with Porcicoll positively modifies chromatin structure in boar spermatozoa. Theriogenology. 2023;201 :95-105. doi: 10.1016/j.theriogenology.2023.02.017. PubMed PMID:36857978 .
  8. Fernández-Alegre, E, Lacalle, E, Soriano-Úbeda, C, González-Montaña, JR, Domínguez, JC, Casao, A et al.. Bos taurus and Cervus elaphus as Non-Seasonal/Seasonal Models for the Role of Melatonin Receptors in the Spermatozoon. Int J Mol Sci. 2022;23 (11):. doi: 10.3390/ijms23116284. PubMed PMID:35682961 PubMed Central PMC9181011.
  9. Fernández-Alegre, E, Lacalle, E, Soriano-Úbeda, C, Carlos Domínguez, J, Casao, A, Martínez-Pastor, F et al.. Melatonin affects red deer spermatozoa motility and physiology in capacitating and non-capacitating conditions. Reprod Domest Anim. 2022;57 Suppl 5 (Suppl 5):82-85. doi: 10.1111/rda.14137. PubMed PMID:35488500 PubMed Central PMC9790586.
  10. Lacalle, E, Consuegra, C, Martínez, CA, Hidalgo, M, Dorado, J, Martínez-Pastor, F et al.. Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts. Animals (Basel). 2022;12 (8):. doi: 10.3390/ani12081012. PubMed PMID:35454259 PubMed Central PMC9031844.
  11. Giaretta, E, Mislei, B, Martínez-Pastor, F, Nesci, S, Spinaci, M, Galeati, G et al.. Use of specific mitochondrial complex inhibitors to investigate mitochondrial involvement on horse sperm motility and ROS production. Res Vet Sci. 2022;147 :12-19. doi: 10.1016/j.rvsc.2022.03.017. PubMed PMID:35397468 .
  12. Piñán, J, Alegre, B, Kirkwood, RN, Soriano-Úbeda, C, Maj, M, Domínguez, JC et al.. Effect of Season and Parity on Reproduction Performance of Iberian Sows Bred with Duroc Semen. Animals (Basel). 2021;11 (11):. doi: 10.3390/ani11113275. PubMed PMID:34828007 PubMed Central PMC8614353.
  13. Piñán, J, Martinez-Pastor, F, Alegre, B, Maj, M, Kirkwood, RN, Domínguez, JC et al.. The Suinfort® Semen Supplement Counters Seasonal Infertility in Iberian Sows. Animals (Basel). 2021;11 (11):. doi: 10.3390/ani11113176. PubMed PMID:34827908 PubMed Central PMC8614245.
  14. Escalera-Valente, F, Alonso, ME, Lomillos-Pérez, JM, Gaudioso-Lacasa, VR, Alonso, AJ, González-Montaña, JR et al.. Blood Biochemical Variables Found in Lidia Cattle after Intense Exercise. Animals (Basel). 2021;11 (10):. doi: 10.3390/ani11102866. PubMed PMID:34679884 PubMed Central PMC8532696.
  15. Martínez-Pastor, F. What is the importance of sperm subpopulations?. Anim Reprod Sci. 2022;246 :106844. doi: 10.1016/j.anireprosci.2021.106844. PubMed PMID:34538510 .
  16. Arregui, L, Martinez-Pastor, F, Arroyo, F, Gosálvez, J. Determining the effects of sperm activation in anuran cloaca on motility and DNA integrity in Epidalea calamita (Bufonidae). Reprod Fertil Dev. 2022;34 (5):438-446. doi: 10.1071/RD21063. PubMed PMID:34399881 .
  17. Mehdipour, M, Daghigh-Kia, H, Najafi, A, Martínez-Pastor, F. Type III antifreeze protein (AFP) improves the post-thaw quality and in vivo fertility of rooster spermatozoa. Poult Sci. 2021;100 (8):101291. doi: 10.1016/j.psj.2021.101291. PubMed PMID:34217904 PubMed Central PMC8260870.
  18. Escalera-Valente, F, Alonso, ME, Lomillos, JM, Gaudioso, VR, Alonso, ÁJ, González-Montaña, JR et al.. Effect of Intense Exercise on Plasma Macrominerals and Trace Elements in Lidia Bulls. Vet Sci. 2021;8 (6):. doi: 10.3390/vetsci8060097. PubMed PMID:34205854 PubMed Central PMC8228837.
  19. Caamaño, JN, Tamargo, C, Parrilla, I, Martínez-Pastor, F, Padilla, L, Salman, A et al.. Post-Thaw Sperm Quality and Functionality in the Autochthonous Pig Breed Gochu Asturcelta. Animals (Basel). 2021;11 (7):. doi: 10.3390/ani11071885. PubMed PMID:34202862 PubMed Central PMC8300257.
  20. Salman, A, Caamaño, JN, Fernández-Alegre, E, Hidalgo, CO, Nadri, T, Tamargo, C et al.. Supplementation of the BIOXcell extender with the antioxidants crocin, curcumin and GSH for freezing bull semen. Res Vet Sci. 2021;136 :444-452. doi: 10.1016/j.rvsc.2021.03.025. PubMed PMID:33812287 .
  21. Álvarez-Rodríguez, M, Martinez-Pastor, F. Molecular Determinants of Seminal Plasma on Sperm Biology and Fertility. Int J Mol Sci. 2021;22 (7):. doi: 10.3390/ijms22073555. PubMed PMID:33808064 PubMed Central PMC8037708.
  22. Lacalle, E, Núñez, A, Fernández-Alegre, E, Crespo-Félez, I, Domínguez, JC, Alonso, ME et al.. Cold-Shock Test Is a Practical Method for Selecting Boar Ejaculates Yielding Appropriate Seminal Plasma for Post-Thawing Supplementation. Animals (Basel). 2021;11 (3):. doi: 10.3390/ani11030871. PubMed PMID:33803834 PubMed Central PMC8003138.
  23. Najafi, A, Daghigh-Kia, H, Martínez-Pastor, F. Poloxamer 188 and hydroxyethyl starch have a cryoprotective synergic effect improving post-thawing quality and fertility of rooster spermatozoa. Anim Reprod Sci. 2021;228 :106738. doi: 10.1016/j.anireprosci.2021.106738. PubMed PMID:33780711 .
  24. Martínez-Pastor, F, Lacalle, E, Martínez-Martínez, S, Fernández-Alegre, E, Álvarez-Fernández, L, Martinez-Alborcia, MJ et al.. Low density Porcicoll separates spermatozoa from bacteria and retains sperm quality. Theriogenology. 2021;165 :28-36. doi: 10.1016/j.theriogenology.2021.02.009. PubMed PMID:33611172 .
  25. Mehdipour, M, Daghigh Kia, H, Martínez-Pastor, F. Poloxamer 188 exerts a cryoprotective effect on rooster sperm and allows decreasing glycerol concentration in the freezing extender. Poult Sci. 2020;99 (11):6212-6220. doi: 10.1016/j.psj.2020.08.041. PubMed PMID:33142539 PubMed Central PMC7647912.
  26. González-Montaña, JR, Escalera-Valente, F, Alonso, AJ, Lomillos, JM, Robles, R, Alonso, ME et al.. Relationship between Vitamin B12 and Cobalt Metabolism in Domestic Ruminant: An Update. Animals (Basel). 2020;10 (10):. doi: 10.3390/ani10101855. PubMed PMID:33053716 PubMed Central PMC7601760.
  27. Lomillos, JM, Alonso, ME. Morphometric Characterization of the Lidia Cattle Breed. Animals (Basel). 2020;10 (7):. doi: 10.3390/ani10071180. PubMed PMID:32668614 PubMed Central PMC7401559.
  28. Olfati, A, Martínez-Pastor, F. RETRACTION NOTE TO: The effects of sericin in recovering spermatogenesis and sexual hormone levels in diabetic rats. Physiol Int. 2019; :1. doi: 10.1556/2060.106.2019.0001. PubMed PMID:32592638 .
  29. Fernández-Alegre, E, Álvarez-Fernández, I, Domínguez, JC, Casao, A, Martínez-Pastor, F. Melatonin Non-Linearly Modulates Bull Spermatozoa Motility and Physiology in Capacitating and Non-Capacitating Conditions. Int J Mol Sci. 2020;21 (8):. doi: 10.3390/ijms21082701. PubMed PMID:32295040 PubMed Central PMC7215461.
  30. Alonso, ME, González-Montaña, JR, Lomillos, JM. Consumers' Concerns and Perceptions of Farm Animal Welfare. Animals (Basel). 2020;10 (3):. doi: 10.3390/ani10030385. PubMed PMID:32120935 PubMed Central PMC7143148.
  31. Ngula, J, Manjarín, R, Martínez-Pastor, F, Alegre, B, Tejedor, I, Brown, T et al.. A novel semen supplement (SuinFort) improves sow fertility after artificial insemination. Anim Reprod Sci. 2019;210 :106193. doi: 10.1016/j.anireprosci.2019.106193. PubMed PMID:31635779 .
  32. Tamargo, C, Hidalgo, CO, Caamaño, JN, Salman, A, Fueyo, C, Arija, C et al.. Assessment of a germplasm bank for the autochthonous cattle breed Asturiana de la Montaña: Extender (Biociphos vs. BIOXCell) affected sperm quality but not field fertility. Reprod Domest Anim. 2019;54 Suppl 4 :90-93. doi: 10.1111/rda.13502. PubMed PMID:31625236 .
  33. Manjarín, R, Kirkwood, RN, Ngula, J, Martinez-Pastor, F, Alegre, B, Domínguez, JC et al.. Effect of Oxytocin, Cloprostenol or Buserelin in Semen Doses on Sow Fertility. Animals (Basel). 2019;9 (10):. doi: 10.3390/ani9100746. PubMed PMID:31569532 PubMed Central PMC6827092.
  34. Olfati, A, Martínez-Pastor, F. The effects of sericin in recovering spermatogenesis and sexual hormone levels in diabetic rats. Physiol Int. 2019; :1-7. doi: 10.1556/2060.106.2019.23. PubMed PMID:31564119 .
  35. González-Montaña, JR, Escalera-Valente, F, Lomillos, JM, Alonso, AJ, Gaudioso, V, Alonso, ME et al.. Relationships between eye fluids and blood values after exercise in lidia cattle: mineral parameters. Pol J Vet Sci. 2019;22 (3):445-455. doi: 10.24425/pjvs.2019.129304. PubMed PMID:31560465 .
  36. González-Montaña, JR, Senís, E, Alonso, AJ, Alonso, ME, Alonso, MP, Domínguez, JC et al.. Some toxic metals (Al, As, Mo, Hg) from cow's milk raised in a possibly contaminated area by different sources. Environ Sci Pollut Res Int. 2019;26 (28):28909-28918. doi: 10.1007/s11356-019-06036-7. PubMed PMID:31385249 .
  37. Nadri, T, Towhidi, A, Zeinoaldini, S, Martínez-Pastor, F, Mousavi, M, Noei, R et al.. Lecithin nanoparticles enhance the cryosurvival of caprine sperm. Theriogenology. 2019;133 :38-44. doi: 10.1016/j.theriogenology.2019.04.024. PubMed PMID:31055160 .
  38. Morrell, JM, Núñez-González, A, Crespo-Félez, I, Martínez-Martínez, S, Martínez Alborcia, MJ, Fernández-Alegre, E et al.. Removal of bacteria from boar semen using a low-density colloid. Theriogenology. 2019;126 :272-278. doi: 10.1016/j.theriogenology.2018.12.028. PubMed PMID:30594102 .
  39. Martínez-Pastor, F, Álvarez, M, Guerra, C, Chamorro, CA, Anel-López, L, de Paz, P et al.. Extender osmolality, glycerol and egg yolk on the cryopreservation of epididymal spermatozoa for gamete banking of the Cantabric Chamois (Rupicapra pyrenaica parva). Theriogenology. 2019;125 :109-114. doi: 10.1016/j.theriogenology.2018.10.022. PubMed PMID:30408702 .
  40. Álvarez-Rodriguez, M, Álvarez, M, Anel-López, L, Guerra, C, Chamorro, CA, Anel, L et al.. Effect of length of time post-mortem on quality and freezing capacity of Cantabric chamois (Rupicapra pyrenaica parva) epididymal spermatozoa. Anim Reprod Sci. 2018;198 :184-192. doi: 10.1016/j.anireprosci.2018.09.018. PubMed PMID:30301620 .
  41. Najafi, A, Taheri, RA, Mehdipour, M, Martínez-Pastor, F, Rouhollahi, AA, Nourani, MR et al.. Improvement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomes. Poult Sci. 2019;98 (1):440-446. doi: 10.3382/ps/pey353. PubMed PMID:30085198 PubMed Central PMC6347126.
  42. Najafi, A, Taheri, RA, Mehdipour, M, Farnoosh, G, Martínez-Pastor, F. Lycopene-loaded nanoliposomes improve the performance of a modified Beltsville extender broiler breeder roosters. Anim Reprod Sci. 2018;195 :168-175. doi: 10.1016/j.anireprosci.2018.05.021. PubMed PMID:29880233 .
  43. Sánchez-Rubio, F, Fernández-Santos, MR, Castro-Vázquez, L, García-Álvarez, O, Maroto-Morales, A, Soler, AJ et al.. Cinnamtannin B-1, a novel antioxidant for sperm in red deer. Anim Reprod Sci. 2018;195 :44-52. doi: 10.1016/j.anireprosci.2018.05.004. PubMed PMID:29776697 .
  44. Ramón, M, Martínez-Pastor, F. Implementation of novel statistical procedures and other advanced approaches to improve analysis of CASA data. Reprod Fertil Dev. 2018;30 (6):860-866. doi: 10.1071/RD17479. PubMed PMID:29681257 .
  45. Norouzirad, R, González-Montaña, JR, Martínez-Pastor, F, Hosseini, H, Shahrouzian, A, Khabazkhoob, M et al.. Lead and cadmium levels in raw bovine milk and dietary risk assessment in areas near petroleum extraction industries. Sci Total Environ. 2018;635 :308-314. doi: 10.1016/j.scitotenv.2018.04.138. PubMed PMID:29665547 .
  46. Crespo-Félez, I, Castañeda-Sampedro, A, Sánchez, DI, Fernández-Alegre, E, Álvarez-Rodríguez, M, Domínguez, JC et al.. Effect of Single Layer Centrifugation Porcicoll (70%, 80% and 90%) or supplementation with reduced glutathione, seminal plasma and bovine serum albumin on frozen-thawed boar sperm. Anim Reprod Sci. 2017;187 :167-173. doi: 10.1016/j.anireprosci.2017.11.002. PubMed PMID:29113727 .
  47. Malo, AF, Martinez-Pastor, F, Garcia-Gonzalez, F, Garde, J, Ballou, JD, Lacy, RC et al.. A father effect explains sex-ratio bias. Proc Biol Sci. 2017;284 (1861):. doi: 10.1098/rspb.2017.1159. PubMed PMID:28855362 PubMed Central PMC5577485.
  48. Ledesma, A, Zalazar, L, Fernández-Alegre, E, Hozbor, F, Cesari, A, Martínez-Pastor, F et al.. Seminal plasma proteins modify the distribution of sperm subpopulations in cryopreserved semen of rams with lesser fertility. Anim Reprod Sci. 2017;184 :44-50. doi: 10.1016/j.anireprosci.2017.06.015. PubMed PMID:28728862 .
  49. Hall, DR, Farman, D, Domínguez, JC, Pajares, JA. Female Sex Pheromone of the Cone Moth, Dioryctria mendacella: Investigation of Synergism between Type I and Type II Pheromone Components. J Chem Ecol. 2017;43 (5):433-442. doi: 10.1007/s10886-017-0846-8. PubMed PMID:28500568 .
  50. Gómez-Seco, C, Alegre, B, Martínez-Pastor, F, Prieto, JG, González-Montaña, JR, Alonso, ME et al.. Evolution of the corpus luteum volume determined ultrasonographically and its relation to the plasma progesterone concentration after artificial insemination in pregnant and non-pregnant dairy cows. Vet Res Commun. 2017;41 (3):183-188. doi: 10.1007/s11259-017-9685-x. PubMed PMID:28337578 .
  51. Karimi, R, Towhidi, A, Zeinoaldini, S, Rezayazdi, K, Mousavi, M, Safari, H et al.. Effects of supplemental conjugated linoleic acids (CLA) on fresh and post-thaw sperm quality of Holstein bulls. Reprod Domest Anim. 2017;52 (3):459-467. doi: 10.1111/rda.12932. PubMed PMID:28176378 .
  52. Maroto-Morales, A, García-Álvarez, O, Ramón, M, Martínez-Pastor, F, Fernández-Santos, MR, Soler, AJ et al.. Current status and potential of morphometric sperm analysis. Asian J Androl. 2016;18 (6):863-870. doi: 10.4103/1008-682X.187581. PubMed PMID:27678465 PubMed Central PMC5109877.
  53. Ledesma, A, Fernández-Alegre, E, Cano, A, Hozbor, F, Martínez-Pastor, F, Cesari, A et al.. Seminal plasma proteins interacting with sperm surface revert capacitation indicators in frozen-thawed ram sperm. Anim Reprod Sci. 2016;173 :35-41. doi: 10.1016/j.anireprosci.2016.08.007. PubMed PMID:27570190 .
  54. Fernández-Gago, R, Álvarez-Rodríguez, M, Alonso, ME, González, JR, Alegre, B, Domínguez, JC et al.. Thawing boar semen in the presence of seminal plasma improves motility, modifies subpopulation patterns and reduces chromatin alterations. Reprod Fertil Dev. 2017;29 (8):1576-1584. doi: 10.1071/RD15530. PubMed PMID:27543989 .
  55. Del Olmo, E, García-Álvarez, O, Maroto-Morales, A, Ramón, M, Iniesta-Cuerda, M, Martinez-Pastor, F et al.. Oestrous sheep serum balances ROS levels to supply in vitro capacitation of ram spermatozoa. Reprod Domest Anim. 2016;51 (5):743-50. doi: 10.1111/rda.12741. PubMed PMID:27491678 .
  56. González-Arto, M, Vicente-Carrillo, A, Martínez-Pastor, F, Fernández-Alegre, E, Roca, J, Miró, J et al.. Melatonin receptors MT1 and MT2 are expressed in spermatozoa from several seasonal and nonseasonal breeder species. Theriogenology. 2016;86 (8):1958-68. doi: 10.1016/j.theriogenology.2016.06.016. PubMed PMID:27448693 .
  57. Sánchez-García, C, Alonso, ME, Tizado, EJ, Pérez, JA, Armenteros, JA, Gaudioso, VR et al.. Anti-predator behaviour of adult red-legged partridge (Alectoris rufa) tutors improves the defensive responses of farm-reared broods. Br Poult Sci. 2016;57 (3):306-16. doi: 10.1080/00071668.2016.1162283. PubMed PMID:26955894 .
  58. Del Olmo, E, García-Álvarez, O, Maroto-Morales, A, Ramón, M, Jiménez-Rabadán, P, Iniesta-Cuerda, M et al.. Estrous sheep serum enables in vitro capacitation of ram spermatozoa while preventing caspase activation. Theriogenology. 2016;85 (2):351-60. doi: 10.1016/j.theriogenology.2015.09.005. PubMed PMID:26474680 .
  59. Mata-Campuzano, M, Soleilhavoup, C, Tsikis, G, Martinez-Pastor, F, de Graaf, SP, Druart, X et al.. Motility of liquid stored ram spermatozoa is altered by dilution rate independent of seminal plasma concentration. Anim Reprod Sci. 2015;162 :31-6. doi: 10.1016/j.anireprosci.2015.09.004. PubMed PMID:26421370 .
  60. Pérez, JA, Sánchez-García, C, Díez, C, Bartolomé, DJ, Alonso, ME, Gaudioso, VR et al.. Are parent-reared red-legged partridges (Alectoris rufa) better candidates for re-establishment purposes?. Poult Sci. 2015;94 (10):2330-8. doi: 10.3382/ps/pev210. PubMed PMID:26362976 .
  61. Manjarín, R, Cassar, G, Friendship, RM, Garcia, JC, Dominguez, JC, Kirkwood, RN et al.. Effect of additional human chorionic gonadotrophin (hCG) on follicular growth and ovulation in gonadotrophin-treated gilts. Can J Vet Res. 2015;79 (3):210-3. . PubMed PMID:26130853 PubMed Central PMC4445513.
  62. Casao, A, Mata-Campuzano, M, Ordás, L, Cebrián-Pérez, JA, Muiño-Blanco, T, Martínez-Pastor, F et al.. Cleaved PARP-1, an Apoptotic Marker, can be Detected in Ram Spermatozoa. Reprod Domest Anim. 2015;50 (4):688-91. doi: 10.1111/rda.12549. PubMed PMID:26031316 .
  63. Hidalgo, DM, Cassar, G, Manjarin, R, Dominguez, JC, Friendship, RM, Kirkwood, RN et al.. Relationship between vaginal mucus conductivity and time of ovulation in weaned sows. Can J Vet Res. 2015;79 (2):151-4. . PubMed PMID:25852232 PubMed Central PMC4365708.
  64. Mata-Campuzano, M, Álvarez-Rodríguez, M, Álvarez, M, Tamayo-Canul, J, Anel, L, de Paz, P et al.. Post-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extender. Theriogenology. 2015;83 (4):520-8. doi: 10.1016/j.theriogenology.2014.10.018. PubMed PMID:25499089 .
  65. Mata-Campuzano, M, Álvarez-Rodríguez, M, Tamayo-Canul, J, López-Urueña, E, de Paz, P, Anel, L et al.. Refrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: effect of temperature, extender and storage time. Anim Reprod Sci. 2014;151 (3-4):137-47. doi: 10.1016/j.anireprosci.2014.10.006. PubMed PMID:25458318 .
  66. Gallego, V, Vílchez, MC, Peñaranda, DS, Pérez, L, Herráez, MP, Asturiano, JF et al.. Subpopulation pattern of eel spermatozoa is affected by post-activation time, hormonal treatment and the thermal regimen. Reprod Fertil Dev. 2015;27 (3):529-43. doi: 10.1071/RD13198. PubMed PMID:25402273 .
  67. Najafi, A, Najafi, MH, Zanganeh, Z, Sharafi, M, Martinez-Pastor, F, Adeldust, H et al.. Cryopreservation of ram semen in extenders containing soybean lecithin as cryoprotectant and hyaluronic acid as antioxidant. Reprod Domest Anim. 2014;49 (6):934-40. doi: 10.1111/rda.12405. PubMed PMID:25219460 .
  68. Hidalgo, DM, Friendship, RM, Greiner, L, Manjarín, R, Amezcua, MR, Domínguez, JC et al.. Influence of gonadotrophin-induced first oestrus on gilt fertility. Reprod Domest Anim. 2014;49 (6):899-902. doi: 10.1111/rda.12390. PubMed PMID:25125139 .
  69. Hidalgo, DM, Friendship, RM, Greiner, L, Manjarin, R, Amezcua, MR, Dominguez, JC et al.. Influence of lactation length and gonadotrophins administered at weaning on fertility of primiparous sows. Anim Reprod Sci. 2014;149 (3-4):245-8. doi: 10.1016/j.anireprosci.2014.06.034. PubMed PMID:25064560 .
  70. Najafi, A, Kia, HD, Mohammadi, H, Najafi, MH, Zanganeh, Z, Sharafi, M et al.. Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender. Cryobiology. 2014;69 (1):68-73. doi: 10.1016/j.cryobiol.2014.05.004. PubMed PMID:24854868 .
  71. Fernández-Gago, R, Domínguez, JC, Martínez-Pastor, F. Seminal plasma applied post-thawing affects boar sperm physiology: a flow cytometry study. Theriogenology. 2013;80 (4):400-10. doi: 10.1016/j.theriogenology.2013.05.003. PubMed PMID:23756043 .
  72. Robles, V, Martínez-Pastor, F, Petroni, G, Riesco, MF, Bozzano, A, Villanueva, R et al.. Cryobiology of cephalopod (Illex coindetii) spermatophores. Cryobiology. 2013;66 (3):288-94. doi: 10.1016/j.cryobiol.2013.03.005. PubMed PMID:23523625 .
  73. Najafi, A, Zhandi, M, Towhidi, A, Sharafi, M, Akbari Sharif, A, Khodaei Motlagh, M et al.. Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology. 2013;66 (3):275-82. doi: 10.1016/j.cryobiol.2013.03.002. PubMed PMID:23500077 .
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  75. Alvarez-Rodríguez, M, Alvarez, M, Anel-López, L, Martínez-Rodríguez, C, Martínez-Pastor, F, Borragan, S et al.. The antioxidant effects of soybean lecithin- or low-density lipoprotein-based extenders for the cryopreservation of brown-bear (Ursus arctos) spermatozoa. Reprod Fertil Dev. 2013;25 (8):1185-93. doi: 10.1071/RD12181. PubMed PMID:23312130 .
  76. Alvarez-Rodríguez, M, Alvarez, M, Borragan, S, Martinez-Pastor, F, Holt, WV, Fazeli, A et al.. The addition of heat shock protein HSPA8 to cryoprotective media improves the survival of brown bear (Ursus arctos) spermatozoa during chilling and after cryopreservation. Theriogenology. 2013;79 (3):541-50. doi: 10.1016/j.theriogenology.2012.11.006. PubMed PMID:23237481 .
  77. Alvarez, M, Nicolas, M, Borragán, S, Lopez-Urueña, E, Anel-López, L, Martinez-Pastor, F et al.. The percentage of spermatozoa lost during the centrifugation of brown bear (Ursus arctos) ejaculates is associated with some spermatozoa quality and seminal plasma characteristics. Anim Reprod Sci. 2012;135 (1-4):113-21. doi: 10.1016/j.anireprosci.2012.09.009. PubMed PMID:23084761 .
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  80. Mata-Campuzano, M, Alvarez-Rodríguez, M, del Olmo, E, Fernández-Santos, MR, Garde, JJ, Martínez-Pastor, F et al.. Quality, oxidative markers and DNA damage (DNA) fragmentation of red deer thawed spermatozoa after incubation at 37 °C in presence of several antioxidants. Theriogenology. 2012;78 (5):1005-19. doi: 10.1016/j.theriogenology.2011.12.018. PubMed PMID:22818091 .
  81. Prieto, R, Sánchez-García, C, Alonso, ME, Rodríguez, PL, Gaudioso, VR. Do pairing systems improve welfare of captive Red-Legged partridges (Alectoris rufa) in laying cages?. Poult Sci. 2012;91 (8):1751-8. doi: 10.3382/ps.2011-01677. PubMed PMID:22802164 .
  82. Álvarez, M, Tamayo-Canul, J, Martínez-Rodríguez, C, López-Urueña, E, Gomes-Alves, S, Anel, L et al.. Specificity of the extender used for freezing ram sperm depends of the spermatozoa source (ejaculate, electroejaculate or epididymis). Anim Reprod Sci. 2012;132 (3-4):145-54. doi: 10.1016/j.anireprosci.2012.05.006. PubMed PMID:22673394 .
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  84. Ramón, M, Martínez-Pastor, F, García-Álvarez, O, Maroto-Morales, A, Soler, AJ, Jiménez-Rabadán, P et al.. Taking advantage of the use of supervised learning methods for characterization of sperm population structure related with freezability in the Iberian red deer. Theriogenology. 2012;77 (8):1661-72. doi: 10.1016/j.theriogenology.2011.12.011. PubMed PMID:22341709 .
  85. Martínez-Rodríguez, C, Alvarez, M, Ordás, L, Chamorro, CA, Martinez-Pastor, F, Anel, L et al.. Evaluation of ram semen quality using polyacrylamide gel instead of cervical mucus in the sperm penetration test. Theriogenology. 2012;77 (8):1575-86. doi: 10.1016/j.theriogenology.2011.11.026. PubMed PMID:22289220 .
  86. Alvarez, M, Tamayo-Canul, J, Anel, E, Boixo, JC, Mata-Campuzano, M, Martinez-Pastor, F et al.. Sperm concentration at freezing affects post-thaw quality and fertility of ram semen. Theriogenology. 2012;77 (6):1111-8. doi: 10.1016/j.theriogenology.2011.10.013. PubMed PMID:22192392 .
  87. Nicolas, M, Alvarez, M, Borragán, S, Martinez-Pastor, F, Chamorro, CA, Alvarez-Rodriguez, M et al.. Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa. Theriogenology. 2012;77 (6):1119-28. doi: 10.1016/j.theriogenology.2011.10.016. PubMed PMID:22154477 .
  88. Tamayo-Canul, J, Alvarez, M, Mata-Campuzano, M, Alvarez-Rodríguez, M, de Paz, P, Anel, L et al.. Effect of storage method and extender osmolality in the quality of cryopreserved epididymal ram spermatozoa. Anim Reprod Sci. 2011;129 (3-4):188-99. doi: 10.1016/j.anireprosci.2011.11.003. PubMed PMID:22137791 .
  89. Riesco, MF, Martínez-Pastor, F, Chereguini, O, Robles, V. Evaluation of zebrafish (Danio rerio) PGCs viability and DNA damage using different cryopreservation protocols. Theriogenology. 2012;77 (1):122-30, 130.e1-2. doi: 10.1016/j.theriogenology.2011.07.024. PubMed PMID:21872308 .
  90. Gaudioso, VR, Sánchez-García, C, Pérez, JA, Rodríguez, PL, Armenteros, JA, Alonso, ME et al.. Does early antipredator training increase the suitability of captive red-legged partridges (Alectoris rufa) for releasing?. Poult Sci. 2011;90 (9):1900-8. doi: 10.3382/ps.2011-01430. PubMed PMID:21844253 .
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  92. Tamayo-Canul, J, Alvarez, M, López-Urueña, E, Nicolas, M, Martinez-Pastor, F, Anel, E et al.. Undiluted or extended storage of ram epididymal spermatozoa as alternatives to refrigerating the whole epididymes. Anim Reprod Sci. 2011;126 (1-2):76-82. doi: 10.1016/j.anireprosci.2011.04.011. PubMed PMID:21601384 .
  93. Gaudioso, VR, Pérez, JA, Sánchez-García, C, Armenteros, JA, Lomillos, JM, Alonso, ME et al.. Isolation from predators: a key factor in the failed release of farmed red-legged partridges (Alectoris rufa) to the wild?. Br Poult Sci. 2011;52 (2):155-62. doi: 10.1080/00071668.2010.549668. PubMed PMID:21491237 .
  94. Alvarez-Rodríguez, M, Alvarez, M, Gomes-Alves, S, Borragan, S, Martinez-Pastor, F, de Paz, P et al.. Quality of frozen-thawed semen in brown bear is not affected by timing of glycerol addition. Theriogenology. 2011;75 (8):1561-5. doi: 10.1016/j.theriogenology.2010.12.009. PubMed PMID:21295827 .
  95. Martínez-Pastor, F, Tizado, EJ, Garde, JJ, Anel, L, de Paz, P. Statistical Series: Opportunities and challenges of sperm motility subpopulation analysis. Theriogenology. 2011;75 (5):783-95. doi: 10.1016/j.theriogenology.2010.11.034. PubMed PMID:21220164 .
  96. Domínguez-Rebolledo, AE, Martínez-Pastor, F, Bisbal, AF, Ros-Santaella, JL, García-Álvarez, O, Maroto-Morales, A et al.. Response of thawed epididymal red deer spermatozoa to increasing concentrations of hydrogen peroxide, and importance of individual male variability. Reprod Domest Anim. 2011;46 (3):393-403. doi: 10.1111/j.1439-0531.2010.01677.x. PubMed PMID:20678089 .
  97. Martínez-Pastor, F, Mata-Campuzano, M, Alvarez-Rodríguez, M, Alvarez, M, Anel, L, de Paz, P et al.. Probes and techniques for sperm evaluation by flow cytometry. Reprod Domest Anim. 2010;45 Suppl 2 :67-78. doi: 10.1111/j.1439-0531.2010.01622.x. PubMed PMID:20591067 .
  98. Anel, L, Alvarez, M, Anel, E, Martinez-Pastor, F, Martinez, F, Chamorro, C et al.. Evaluation of three different extenders for use in emergency salvaging of epididymal spermatozoa from a Cantabric brown bear. Reprod Domest Anim. 2011;46 (1):e85-90. doi: 10.1111/j.1439-0531.2010.01646.x. PubMed PMID:20546173 .
  99. Malo, AF, Martinez-Pastor, F, Alaks, G, Dubach, J, Lacy, RC. Effects of genetic captive-breeding protocols on sperm quality and fertility in the white-footed mouse. Biol Reprod. 2010;83 (4):540-8. doi: 10.1095/biolreprod.110.085316. PubMed PMID:20519695 .
  100. Anel, L, Gomes-Alves, S, Alvarez, M, Borragan, S, Anel, E, Nicolas, M et al.. Effect of basic factors of extender composition on post-thawing quality of brown bear electroejaculated spermatozoa. Theriogenology. 2010;74 (4):643-51. doi: 10.1016/j.theriogenology.2010.03.004. PubMed PMID:20494423 .
  101. Domínguez-Rebolledo, AE, Fernández-Santos, MR, Bisbal, A, Ros-Santaella, JL, Ramón, M, Carmona, M et al.. Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments. Reprod Fertil Dev. 2010;22 (5):856-70. doi: 10.1071/RD09197. PubMed PMID:20450838 .
  102. Domínguez-Rebolledo, ÁE, Martínez-Pastor, F, Fernández-Santos, MR, del Olmo, E, Bisbal, A, Ros-Santaella, JL et al.. Comparison of the TBARS assay and BODIPY C11 probes for assessing lipid peroxidation in red deer spermatozoa. Reprod Domest Anim. 2010;45 (6):e360-8. doi: 10.1111/j.1439-0531.2009.01578.x. PubMed PMID:20113445 .
  103. Martínez-Pastor, F. Use of misleading statistics in method comparison analyses. J Androl. 2010;31 (4):323. doi: 10.2164/jandrol.109.009704. PubMed PMID:20075415 .
  104. Maroto-Morales, A, Ramón, M, García-Alvarez, O, Soler, AJ, Esteso, MC, Martínez-Pastor, F et al.. Characterization of ram (Ovis aries) sperm head morphometry using the Sperm-Class Analyzer. Theriogenology. 2010;73 (4):437-48. doi: 10.1016/j.theriogenology.2009.10.003. PubMed PMID:20018357 .
  105. Pérez, JA, Alonso, ME, Prieto, R, Bartolomé, D, Gaudioso, DV. Influence of the breeding system on the escape response of red-legged partridges (Alectoris rufa). Poult Sci. 2010;89 (1):5-12. doi: 10.3382/ps.2009-00170. PubMed PMID:20008796 .
  106. Garcia, JC, Dominguez, JC, Pena, FJ, Alegre, B, Gonzalez, R, Castro, MJ et al.. Thawing boar semen in the presence of seminal plasma: Effects on sperm quality and fertility. Anim Reprod Sci. 2010;119 (1-2):160-5. doi: 10.1016/j.anireprosci.2009.11.001. PubMed PMID:19945234 .
  107. Domínguez-Rebolledo, AE, Fernández-Santos, MR, García-Alvarez, O, Maroto-Morales, A, Garde, JJ, Martínez-Pastor, F et al.. Washing increases the susceptibility to exogenous oxidative stress in red deer spermatozoa. Theriogenology. 2009;72 (8):1073-84. doi: 10.1016/j.theriogenology.2009.06.027. PubMed PMID:19733389 .
  108. García-Alvarez, O, Maroto-Morales, A, Martínez-Pastor, F, Garde, JJ, Ramón, M, Fernández-Santos, MR et al.. Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: electroejaculation and postmortem collection. Theriogenology. 2009;72 (2):160-8. doi: 10.1016/j.theriogenology.2009.02.002. PubMed PMID:19349069 .
  109. Castro-González, D, Alvarez, M, Muro, J, Esteso, MC, de Paz, P, Anel, L et al.. The acidic probe LysoSensor is not useful for acrosome evaluation of cryopreserved ram spermatozoa. Reprod Domest Anim. 2010;45 (2):363-7. doi: 10.1111/j.1439-0531.2008.01333.x. PubMed PMID:19210665 .
  110. Pérez-Cerezales, S, Martínez-Páramo, S, Cabrita, E, Martínez-Pastor, F, de Paz, P, Herráez, MP et al.. Evaluation of oxidative DNA damage promoted by storage in sperm from sex-reversed rainbow trout. Theriogenology. 2009;71 (4):605-13. doi: 10.1016/j.theriogenology.2008.09.057. PubMed PMID:19117601 .
  111. Martínez-Pastor, F, Aisen, E, Fernández-Santos, MR, Esteso, MC, Maroto-Morales, A, García-Alvarez, O et al.. Reactive oxygen species generators affect quality parameters and apoptosis markers differently in red deer spermatozoa. Reproduction. 2009;137 (2):225-35. doi: 10.1530/REP-08-0357. PubMed PMID:19028926 .
  112. García-Alvarez, O, Maroto-Morales, A, Martínez-Pastor, F, Fernández-Santos, MR, Esteso, MC, Pérez-Guzmán, MD et al.. Heterologous in vitro fertilization is a good procedure to assess the fertility of thawed ram spermatozoa. Theriogenology. 2009;71 (4):643-50. doi: 10.1016/j.theriogenology.2008.09.036. PubMed PMID:18996578 .
  113. Esteso, MC, Fernández-Santos, MR, Soler, AJ, Montoro, V, Martínez-Pastor, F, Garde, JJ et al.. Identification of sperm-head morphometric subpopulations in iberian red deer epididymal sperm samples. Reprod Domest Anim. 2009;44 (2):206-11. doi: 10.1111/j.1439-0531.2007.01029.x. PubMed PMID:18992078 .
  114. Fernández-Santos, MR, Domínguez-Rebolledo, AE, Esteso, MC, Garde, JJ, Martínez-Pastor, F. Refrigerated storage of red deer epididymal spermatozoa in the epididymis, diluted and with vitamin C supplementation. Reprod Domest Anim. 2009;44 (2):212-20. doi: 10.1111/j.1439-0531.2007.01032.x. PubMed PMID:18992076 .
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  117. Anel, L, Alvarez, M, Martínez-Pastor, F, Gomes, S, Nicolás, M, Mata, M et al.. Sperm cryopreservation in brown bear (Ursus arctos): preliminary aspects. Reprod Domest Anim. 2008;43 Suppl 4 :9-17. doi: 10.1111/j.1439-0531.2008.01248.x. PubMed PMID:18803752 .
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  119. Martínez-Pastor, F, Fernández-Santos, MR, del Olmo, E, Domínguez-Rebolledo, AE, Esteso, MC, Montoro, V et al.. Mitochondrial activity and forward scatter vary in necrotic, apoptotic and membrane-intact spermatozoan subpopulations. Reprod Fertil Dev. 2008;20 (5):547-56. doi: 10.1071/rd08002. PubMed PMID:18577351 .
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  121. Martínez-Pastor, F, Cabrita, E, Soares, F, Anel, L, Dinis, MT. Multivariate cluster analysis to study motility activation of Solea senegalensis spermatozoa: a model for marine teleosts. Reproduction. 2008;135 (4):449-59. doi: 10.1530/REP-07-0376. PubMed PMID:18367506 .
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