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Las condiciones más relevantes (Art. 2 de la convocatoria) son:
Si te interesa, ponte en contacto con nosotros. Actualmente tenemos en marcha estas líneas, bajo la supervisión de los doctores F. Martínez Pastor y C. Soriano Úbeda:
We carried out a study for testing if seminal plasma (SP) from different boars could affect sperm quality of frozen-thawed semen depending on the boar in collaboration with Topigs-Norsvin study center (León). This study obtained a publication in Animals (https://doi.org/10.3390/ani11030871).
The study was performed to verify the effect of adding SP on post-thaw sperm functions. Then, firstly this study evaluated if a simple cold-shock test (CST) could allow to easily identify the most suitable ejaculates for obtaining SP from the higher quality (SPr, cold-shock-resistant) and lower quality (SPs, cold-shock-sensitive) after the CST. Both SP were incubated with frozen/thawed boar sperm, and analyzed at different times, to check the effects respect the control in some sperm quality parameters as motility, viability and chromatin structure. Our results support a stimulatory role of SP on thawed semen, with additional benefits from SPr. The effect of SP and especially SPr after artificial insemination (AI) should be tested since CST could be a practical test for selecting suitable ejaculates in AI centers. This study was part of our ongoing research line “Increasing the reproductive efficiency of pig breeding through the application of advanced techniques for sperm chromatin analysis” supported by the Spanish Science and Innovation Ministry (RTI2018-095183-B-I00) and the Education Council of Junta of Castilla y León (LE023P20).
We have collaborated with Dr. Heriberto Rodríguez-Martínez and Dr. Manuel Álvarez-Rodríguez at Linköping University (Sweden) on a study checking changes in the relative abundance of mRNA transcripts of some calcium channels before and after in vitro capacitation triggered by bicarbonate, in boar sperm. Our PhD, Estíbaliz Lacalle, stayed at their lab doing some experiments about this item. This study merited a paper in Animals journal in April 2022 (doi:10.3390/ani12081012).
In this paper, we report the changes on CatSper channels and Canonical Transient Potential Channels (TRPC) during in vitro boar sperm capacitation by bicarbonate ions. Our results indicate that in vitro capacitation using bicarbonate elicits an increase in the relative abundance of mRNA transcripts of almost all studied Ca2+ channels, except CatSper-δ and TRPC1, which were significantly reduced. These findings, in our opinion, open new avenues of research to identify the specificic role of each channel in boar sperm capacitation, and elucidate the physiological meaning of the changes of sperm mRNA cargo.
Regarding the possibility of using changes in the abundance of mRNA transcripts after in vitro capacitation as a new biomarker, we consider that it could be a useful tool to evaluate the overall quality status in the commercial doses, after further analysis on how changes in sperm mRNA abundance could be linked, for example, to small changes in this hyperprolific animal model. This collaboration, and the stay of Estíbaliz in Sweden, was part of our ongoing research line “Increasing the reproductive efficiency of pig breeding through the application of advanced techniques for sperm chromatin analysis” supported by the Spanish Science and Innovation Ministry (RTI2018-095183-B-100) and the Education Council of Junta of Castilla y León (LE023P20).
Las condiciones más relevantes (Art. 2 de la convocatoria) son:
Si te interesa, ponte en contacto con nosotros. Actualmente tenemos en marcha estas líneas, bajo la supervisión de los doctores F. Martínez Pastor y C. Soriano Úbeda:
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#bianorbiotech La exposición del padre a extracto de cannabis altera el metiloma de la descendencia, estudio en ratas eneticsandchromatin.biomedcen… 
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Tenemos un contrato postdoc para 6-7 meses en nuestro grupo.
Póster presentado al ICAR 2022.
El contrato se enmarca en un proyecto para estudiar la cromatina espermática, técnicas avanzadas enfocadas a inmunofluorescencia y citometría de flujo. No es imprescindible experiencia en esas técnicas, pero sí en biología celular y molecular en general.
Se valorará experiencia en laboratorio, trabajo en biología del espermatozoide, capacidad para publicar, dirigir estudiantes, etc. Nuestra intención es que la persona que contratemos aproveche al máximo esos meses para su CV: publicaciones, comunicaciones y dirección de estudiantes.
Las condiciones del proyecto y los detalles de los contratos (éste es un contrato parcial por cese) están detallados en https://lnkd.in/gUWznnS